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KMID : 0364219860290040283
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Korean Journal of Zoology
1986 Volume.29 No. 4 p.283 ~ p.293
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Affinity Purification of Human Alpha-Fetoprotein
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Kang Shin-Sung
Bang Ok-Sun
Park Tae-Kyu
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Abstract
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For the preliminary step to make and characterize the monoclonal antibodies of human alpha-fetoprotein (AFP) was purified from 534g of human fetal tissues through the procedures of tissue extraction, DEAE-cellulose, concanavalin A-Sepharose, Cibacron Blue F3GA-agarose and immunoadsorbent affinity chromatography. The isolated AFP preparation showed a single band on polyacrylamide gel electrophoresis and a single precipitin are against rabbit anti-human cord serum and anti-human AFP on immunoelectrophoresis. Our AFP also displayed a single band on SDS-polyacrylamide gel electrophoresis. The recovery of AFP was 8.76mg total.
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